Seminar – Andrew Zydney
Andrew L. Zydney
The Pennsylvania State University
Purification of DNA for Gene Therapy and Vaccines – New Opportunities for Membrane Technology
There is growing interest in using DNA for gene therapy and DNA-based vaccines. Existing methods for DNA purification are inadequate for large-scale commercial production. This talk examines the possibility of using membrane ultrafiltration for the purification of supercoiled plasmid DNA. DNA transmission during ultrafiltration is a strong function of filtrate flux due to flow-induced elongation of the plasmid associated with the converging flow into the membrane pores. Transmission of the open circular DNA was significantly less than that of the supercoiled plasmid, while transmission of the linear DNA was considerably enhanced due to differences in conformational flexibility. New opportunities for enhancing DNA purification were identified using backpulsing to reduce fouling and using novel membranes with controlled pore morphology to pre-stretch the DNA in the elongation flow. The results clearly demonstrate the potential of using membrane systems for commercial-scale purification of DNA.
Dr. Andrew L. Zydney is currently Distinguished Professor of Chemical Engineering at The Pennsylvania State University, having served as Head of that Department from 2004-2014. Professor Zydney received his B.S. in Chemical Engineering from Yale in 1980 and his Ph.D. from M.I.T. in 1985. He was a faculty member in the Chemical Engineering Department at the University of Delaware from 1985 – 2001. Professor Zydney’s research is focused on membrane science and technology, with a particular emphasis on bioseparations and the purification of high value biological products. He has published more than 200 articles on these topics, including invited contributions to the Encyclopedia of Bioprocess Technology and the Handbook of Biomedical Engineering. He is the most recent recipient of the Excellence in Biological Engineering Publications Award from the AIChE. Professor Zydney is the Editor-in-Chief of the Journal of Membrane Science, and he serves on the Editorial Boards for Separation and Purification Reviews, Separation Science and Technology, Current Opinion in Chemical Engineering, and Biotechnology and Bioengineering. He served as President of the North American Membrane Society in 2002 – 2003, and was a member of the Board of Directors for 9 years. He was elected a Fellow of the American Institute of Medical and Biological Engineers, the American Institute of Chemical Engineers, and the American Association for the Advancement of Science. Dr. Zydney has received Excellence in Teaching Awards from the University of Delaware and the Penn State Engineering Alumni Society, and he is a past recipient of the Distinguished Teacher Award and the Outstanding Young Faculty Award from the American Society of Engineering Education.
Dr. Fayang Qiu – Special Seminar Series
Affiliation: Guangzhou Institutes of Biomedicine and Health
Title: “An Achievable Goal for Total Synthesis”
Hosted by: Dr. Fraenkel
Dr. Steven Cundiff – Analytical/Physical Seminar
Dr. Viktor Zhdankin – Organic and Inorganic Seminar Series
Affiliation: University of Minnesota Duluth
Title: “Hypervalent Iodine Chemistry”
Hosted by: Dr. Nagib
Dr. Zach Shultz – Special Seminar
Colloquium – Lisa Everett(University of Wisconsin) Physics Beyond the Standard Model in the LHC Era
Particle physics has entered an exciting and data-rich era in which the physics of the Tera-electron volt scale is being probed at an unprecedented level at the Large Hadron Collider. To date, this exploration has shown the continued triumph of the Standard Model of particle physics in providing a spectacularly successful description of nature — and as such, has placed new facets on the issue of how the Standard Model can be extended to a more complete, fundamental theory. In this talk, I will provide an overview of the current status of the LHC results and their implications for physics beyond the Standard Model, with an emphasis on the issue of Higgs sector naturalness.
CAR Seminar Series: Ke Pan, graduate student, Department of Mechanical and Aerospace Engineering / Athar Hanif, visiting scholar, Mohammad Ali Jinnah University
Presenter: Ke Pan, graduate student, Department of Mechanical and Aerospace Engineering / Athar Hanif, visiting scholar, Mohammad Ali Jinnah University
Topics: “Reduced-order modeling of lithium transport in silicon anodes” (Pan) / “Estimation of Thermally de-rated Torque of an HEV Drive using Robust LPV Observer” (Hanif)
Join faculty, staff, visiting scholars and graduate students to learn about various automotive engineering topics during this weekly seminar series in spring semester 2017. Held in the classroom/room 198 at the Center for Automotive Research. Follow the conversation online: #CARSeminarSeries.
Interprofessional Innovation Symposium
April 12, 2017
4:00 pm — 6:00 pm
The Performance Hall at the Ohio Union
4:00 – 4:30 p.m. – Networking Reception
4:30 – 5:30 p.m. – Speaker Panel
5:30 – 6:00 p.m. – Questions from the audience
Please join us for an engaging conversation focused on the commercialization of academic research. Learn from health care innovators who took the journey from academic research to successful commercialization and founded their own company along the way. Speakers include Dr. Kathy Bowles, PhD, RN, of the University of Pennsylvania, and Eric Heil, CEO, co-founders of RightCare Solutions, a pioneering medical technology company. Heil developed Dr. Bowles’ research into software while an engineering major at the University of Pennsylvania as part of his senior project, and commercialized it as a graduate student at Wharton School of Business. NaviHealth, a subsidiary of Cardinal Health, acquired this software last year. Kevin Taylor, VP Product Management, Global Market Development at Cardinal Health, will also speak.
Please register by March 29th to attend this free symposium.
Co-Sponsored by The Edna Fritz Lectureship Series, The Ohio State University College of Engineering, The Fisher College of Business, OSU ADVANCE and The Ohio State University Technology Commercialization Office.
BME Seminar Series: Dr. Rouzbeh Amini, University of Akron
“Multi-scale Framework for Analysis of Tricuspid Valve Biomechanics “
Mechanics plays a critical role in tissue development, regeneration, and remodeling, as cell-cell interactions and cellmatrix interactions are known to be heavily influenced by changes in the mechanical microenvironment at the extracellular matrix (ECM)/cellular level. In the tricuspid valve (TV), located between the right ventricle and the right atrium in the heart, the leaflets open and close more than three billion times during their lifetime. Thus, TV cells and ECM maintain their homeostasis while subjected to a highly dynamic loading environment. Considering the hierarchy of the living system (i.e. heart, valves, leaflets, and ECM/cellular levels in the case of TV), it is imperative to study biomechanics and mechanobiology using multi-scale approaches. Unfortunately, such multi-scale frameworks do not currently exist, and a main goal of our research lab is to combine experimental techniques and computational simulation to address such major limitations. We are particularly interested in understanding why TV surgery has poor long-term success rate (30% to 40% of patients who undergo surgery have had a recurrence of valve problems). We aim to understand how tricuspid valve repair procedures will affect the valve’s function at the tissue level and at the ECM (micro) level, as we believe that surgical alterations cause changes in tissue stress and tissue microstructure in ways that can eventually lead to failure of the repaired valve.
Dr. Amini completed a Ph.D. in Biomedical Engineering at the University of Minnesota in the field of ocular biomechanics and biotransport in 2010. He then continued his research work on the mechanics of soft tissue as a postdoctoral trainee at the University of Pittsburgh’s Department of Bioengineering, where he held the Ruth L. Kirschstein National Research Service Award (NIH F32). He conducted his postdoctoral research on the biomechanics of cardiac valves. Dr. Amini has served as an assistant professor in the Department of Biomedical Engineering at The University of Akron since August 2013. The overall goal of his research laboratory is to improve human health by studying the multi-scale biomechanics and biotransport in cardiovascular, ocular, and digestive systems. Dr. Amini’s research has been funded by the Akron Children’s Hospital, Firestone Foundation, and American Heart Association.
Dr. Mark Williams – Biochemistry Seminar Series
Affiliation: Northeastern University
Title: “Single molecule DNA-protein interactions: From retroviral restriction to nucleosome stability”
Hosted by: Dr. Musier-Forsyth and Dr. Foster