Seminar – Huimin Zhao
Lowrie Lecture I: Eric Kaler
Seminar – Andrew Zydney
Andrew L. Zydney
The Pennsylvania State University
Purification of DNA for Gene Therapy and Vaccines – New Opportunities for Membrane Technology
There is growing interest in using DNA for gene therapy and DNA-based vaccines. Existing methods for DNA purification are inadequate for large-scale commercial production. This talk examines the possibility of using membrane ultrafiltration for the purification of supercoiled plasmid DNA. DNA transmission during ultrafiltration is a strong function of filtrate flux due to flow-induced elongation of the plasmid associated with the converging flow into the membrane pores. Transmission of the open circular DNA was significantly less than that of the supercoiled plasmid, while transmission of the linear DNA was considerably enhanced due to differences in conformational flexibility. New opportunities for enhancing DNA purification were identified using backpulsing to reduce fouling and using novel membranes with controlled pore morphology to pre-stretch the DNA in the elongation flow. The results clearly demonstrate the potential of using membrane systems for commercial-scale purification of DNA.
Dr. Andrew L. Zydney is currently Distinguished Professor of Chemical Engineering at The Pennsylvania State University, having served as Head of that Department from 2004-2014. Professor Zydney received his B.S. in Chemical Engineering from Yale in 1980 and his Ph.D. from M.I.T. in 1985. He was a faculty member in the Chemical Engineering Department at the University of Delaware from 1985 – 2001. Professor Zydney’s research is focused on membrane science and technology, with a particular emphasis on bioseparations and the purification of high value biological products. He has published more than 200 articles on these topics, including invited contributions to the Encyclopedia of Bioprocess Technology and the Handbook of Biomedical Engineering. He is the most recent recipient of the Excellence in Biological Engineering Publications Award from the AIChE. Professor Zydney is the Editor-in-Chief of the Journal of Membrane Science, and he serves on the Editorial Boards for Separation and Purification Reviews, Separation Science and Technology, Current Opinion in Chemical Engineering, and Biotechnology and Bioengineering. He served as President of the North American Membrane Society in 2002 – 2003, and was a member of the Board of Directors for 9 years. He was elected a Fellow of the American Institute of Medical and Biological Engineers, the American Institute of Chemical Engineers, and the American Association for the Advancement of Science. Dr. Zydney has received Excellence in Teaching Awards from the University of Delaware and the Penn State Engineering Alumni Society, and he is a past recipient of the Distinguished Teacher Award and the Outstanding Young Faculty Award from the American Society of Engineering Education.
Seminar – Ramanan Krishnamoorti
Interim Vice Chancellor/Vice President for Research and Technology Transfer
Chief Energy Officer
University of Houston
Hybrid Nanoparticles: Structure, Dynamics and Properties
Polymer grafted nanoparticles are fascinating materials that bridge the gap between colloidal particles and star-polymers. We have studied the structure and dynamics of highly grafted hybrid nanoparticles in the bulk and in dilute solutions and have elucidated the structure of the polymer chains and the arrangement of the nanoparticles as functions of polymer molecular weight and solvent quality. Extending these to polymer blends wherein the matrix (unattached) chains is attracted to the grafted polymer chains has allowed us to determine the relationship between segment compatibility and polymer dewetting. The consequences of the unique structure and dynamics of these grafted polymer chains on interfacial properties and as mobility control agents for enhanced oil recovery will be discussed in this talk.
Ramanan Krishnamoorti is the Interim vice chancellor/vice president for research and technology transfer and chief energy officer at the University of Houston. He has served as chair of the UH Cullen College of Engineering’s chemical and biomolecular engineering department, associate dean of research for engineering, professor of chemical and biomolecular engineering with affiliated appointments as professor of petroleum engineering and professor of chemistry. Dr. Krishnamoorti obtained his bachelor’s degree in chemical engineering from the Indian Institute of Technology Madras and doctoral degree in chemical engineering from Princeton University in 1994.
The Department of Biomedical Engineering & the Department of Chemical & Biomolecular Engineering Present the Following Seminar:
Speaker: Belinda Hurley, Associate Professor, Research and Education, OSU Libraries
The OSU Libraries spend many millions each year to provide resources that are not freely available online. Belinda Hurley, OSU Libraries’ liaison for Physical Sciences and Engineering, will present an overview of available resources and services to those new to the OSU Libraries system.
Speaker: Anna Balazs, Distinguished Professor, Chemical and Petroleum Engineering
Affiliation: University of Pittsburg
Speaker: Tim Watson, Director of Graduation Services
For graduate students approaching graduation: Come and learn about graduation requirements from the Graduate School.
Details to come.