BME Seminar Series: Dr. Damir Janigro, Flocel Inc.
“Blood-brain barrier in health and diesase”
The blood-brain barrier (BBB) serves to protect the central nervous system (CNS) from damage by exogenous molecules. In doing so, it also can prevent some drugs from reaching their sites of action. A variety of CNS disorders contribute to BBB disruption, and detection of this “opening” can be used for both diagnostic purposes and for determining time periods when drugs can more easily enter the CNS. While expensive and time-consuming imaging techniques are currently used for this purpose, we have devised a method for detecting plasma levels of a blood biomarker of BBB disruption. The relevance of these findings in translational neurosciences will be discussed.
DR. DAMIR JANIGRO, PhD, FAES is the CSO and founder of Flocel Inc. a Professor at CWRU, a member of the World Neurobiology Commission of ILAE, and associate editor for Epilepsia, PLOS among others. He has over 30 years of experience and has received continuous support from the NIH since 1996. He is the inventor of the dynamic in vitro model of the BBB that constituted one of the founding blocks of Flocel’s technology. He discovered S100B as a marker of BBB function and has for many years collaborated with top notch hospitals in the US and Europe to broaden the scope and use of this technology. He recently patented the use of S100B as marker of hemorrhagic transformation in stroke victims undergoing intra-arterial therapies. With his former student, Dr. Nicola Marchi, he received the Morris-Coole award in 2008. He has served on several NIH panels, and has been part of three FDA applications. He served as Chairman for study sections for the American Heart Association and the Department of Defense. He has been associated with neurosurgeons and neurointensivists since his post-doctoral years at the University of Washington (1994-1999). He has published over 150 papers.
BME Seminar Series: Dr. Giuliano Scarcelli, University of Maryland
More details coming soon.
BMEGSA Exchange: Maung Zaw Win
“The Effect of Cellular Architecture on Functional and Mechanical Properties”
Recently, there has been a push towards clinical translation of biomechanical models of tissues by developing patient-specific models to predict disease outcomes. To accomplish this, it is necessary to understand the functional and mechanical properties of all the tissue components, including individual cells. In vasculature, tissues and cells have different structures based on their functional role. The principle goal of this work is to determine how cellular architecture influences function and mechanical properties. To test our hypotheses, we have developed in vitro models to study the relationship between structure and function at the tissue and cellular scale. We have developed microfluidic capture array device (MCAD) technology (Fig. 1) to study cell structure and function in 2D engineered vascular smooth muscle tissue and have developed cellular micro-biaxial stretching (CμBS) microscopy (Fig. 2) to determine single cell mechanical properties. Using MCAD technology we are able to vary initial cell-cell contact during seeding to bias the cellular architecture in confluent vascular smooth muscle tissues. We found that tissues seeded using initially higher cell–cell contact conditions yielded tissues with a more elongated cellular architecture which lead to greater contractile function in engineered tissues. We have also developed CμBS microscopy to determine the anisotropic mechanical properties of individual cells, which we employ to determine the full mechanical description (given by the strain energy density function) of vascular smooth muscle cells. Using our method, we find that smooth muscle cells with native-like architectures are highly anisotropic and can be described by a general strain energy density function based on the actin cytoskeletal organization. Our results suggest that structural organization of cells in organs affect their functional and mechanical properties.
BME Seminar Series: Dr. Rouzbeh Amini, University of Akron
“Multi-scale Framework for Analysis of Tricuspid Valve Biomechanics “
Mechanics plays a critical role in tissue development, regeneration, and remodeling, as cell-cell interactions and cellmatrix interactions are known to be heavily influenced by changes in the mechanical microenvironment at the extracellular matrix (ECM)/cellular level. In the tricuspid valve (TV), located between the right ventricle and the right atrium in the heart, the leaflets open and close more than three billion times during their lifetime. Thus, TV cells and ECM maintain their homeostasis while subjected to a highly dynamic loading environment. Considering the hierarchy of the living system (i.e. heart, valves, leaflets, and ECM/cellular levels in the case of TV), it is imperative to study biomechanics and mechanobiology using multi-scale approaches. Unfortunately, such multi-scale frameworks do not currently exist, and a main goal of our research lab is to combine experimental techniques and computational simulation to address such major limitations. We are particularly interested in understanding why TV surgery has poor long-term success rate (30% to 40% of patients who undergo surgery have had a recurrence of valve problems). We aim to understand how tricuspid valve repair procedures will affect the valve’s function at the tissue level and at the ECM (micro) level, as we believe that surgical alterations cause changes in tissue stress and tissue microstructure in ways that can eventually lead to failure of the repaired valve.
Dr. Amini completed a Ph.D. in Biomedical Engineering at the University of Minnesota in the field of ocular biomechanics and biotransport in 2010. He then continued his research work on the mechanics of soft tissue as a postdoctoral trainee at the University of Pittsburgh’s Department of Bioengineering, where he held the Ruth L. Kirschstein National Research Service Award (NIH F32). He conducted his postdoctoral research on the biomechanics of cardiac valves. Dr. Amini has served as an assistant professor in the Department of Biomedical Engineering at The University of Akron since August 2013. The overall goal of his research laboratory is to improve human health by studying the multi-scale biomechanics and biotransport in cardiovascular, ocular, and digestive systems. Dr. Amini’s research has been funded by the Akron Children’s Hospital, Firestone Foundation, and American Heart Association.
BME Seminar Series: Dr. Lori Setton, Washington University in St. Louis
Dr. Setton is the elected president of the Biomedical Engineering Society (BMES).
“The Stressful Life of the Intervertebral Disc Cell”
Intervertebral disc disorders contribute to pain and disability in millions of affected individuals annually, contributing to low back pain’s ranking as #1 in disease impact in the USA. Pathological processes for resident cells of the intervertebral disc, the nucleus pulposus cells, contribute to premature cell death that can drive loss of intervertebral disc height, tissue destruction and herniation. These nucleus pulposus cells are derived from notochord, unlike the neighboring mesenchymal cells, and are responsible for tissue synthesis and growth in the neonate. With loss of this cell population in the first decades, the intervertebral disc experiences altered disc biochemical composition, cellularity, and material properties that are major contributors to disc pathology. Our laboratory has studied factors that regulate nucleus pulposus cell phenotype and demonstrated an ability to promote biosynthesis and survival through interactions with laminin matrix proteins. We have also advanced knowledge of environmental cues that promote a healthy, biosynthetically active nucleus pulposus cell, factors that can be manipulated to attenuate inflammatory cytokine expression, promote matrix biosynthesis, and control progenitor cell differentiation. In this talk, we will describe our work with engineering substrates and protein-conjugated biomaterials to deliver cells to the disc for regeneration purposes.
Dr. Setton received her B.S.E. from Princeton University in Mechanical and Aerospace Engineering, with M.S. and Ph.D. degrees in Mechanical Engineering from Columbia University. Dr. Setton joined the Department of Biomedical Engineering at Duke University in 1994, where she served as the Bevan Distinguished Professor of Biomedical Engineering and Orthopaedic Surgery. Dr. Setton recently joined the Department of Biomedical Engineering at Washington University to accept the position as Lopata Distinguished Pofessor of Biomedical Engineering & Orthopaedic Surgery.
Dr. Setton’s research focuses on understanding the mechanisms for degeneration and regeneration of soft tissues of the musculoskeletal system. Recent work focuses on development of in situ forming hydrogels for drug delivery and tissue regeneration in the knee joints and spine. She has funded her lab through grants from the NIH, NSF, Whitaker Foundation, Coulter Foundation, OREF, AO Foundation, and research agreements with many corporations.
Dr. Setton has over 180 publications and has licensed several patents for commercial development. She has served on the Editorial Advisory Boards of the Annual Reviews of Biomedical Engineering, Journal of Biomechanical Engineering, Osteoarthritis and Cartilage, and Journal of Biomechanics. Dr. Setton has also served as a permanent member of NIH and NSF study sections, as a consultant to NIH and AAOS, and on the Boards of the Biomedical Engineering Society, Orthopaedic Research Society and World Council on Biomechanics. She is currently serving as President of the Biomedical Engineering Society from 2016-2018. Dr. Setton is a Fellow of the BMES, the AIMBE and has received a PECASE Award, Dean’s Award for Outstanding Research, Graduate Dean’s Award for Excellence in Mentoring, and ASME’s Mow Medal.
A FREE annual conference at the Ohio Union in Columbus, Ohio for Python programmers in and around Ohio, the entire Midwest, maybe even the whole world! There will be many Talks and Tutorials! Please be sure to bring your notebook and laptops and prepare to learn!
What a glimpse of last year’s conference? http://pyvideo.org/events/pyohio-2016.html
Presented by the Construction Systems Management Industry Advisory Council
Women Inspire Construction presented by The Ohio State University Construction Systems Management Industry Advisory Council is an event to gather the construction industry to focus on retention and community. Come to be empowered and join a conversation that leaves you inspired. The construction industry fosters long lasting relationships that lead to professional success. Both men and women are welcome to JOIN THE CONVERSATION!
Meet the panelists:
Nissa Beasley currently works as a Project Manager at TriVersity Construction with 24 years of experience in the construction industry. Nissa specializes in major renovation projects, lab construction and multi-story buildings and schools. She is also TriVersity’s subject matter expert with Phoenix Scheduling Program software and helps train project engineers on the program. Before joining TriVersity, Nissa worked for other high profile construction companies in the region.
Nissa received her B.S. in construction management from the University of Cincinnati. She serves as secretary on the board of NAWIC (National Association of Women in Construction) as well as a participating in its membership committee. Nissa was named NAWIC’s Cincinnati Chapter New Leader in 2017. Through NAWIC, she also volunteers for community events for children including Block Kids and the YWCA’s Rosie’s Girls
Heather Cassady currently work as a Project Manager on the $32M Canopy by Hilton hotel project located in Columbus, Ohio. She has been in the construction industry and with Turner Construction for 16 years. Throughout her years at Turner, Heather’s career has evolved in various management roles, including superintendent, sustainability manager, recruiting leader, and estimating engineer. Heather’s projects in Central Ohio have been in the healthcare, hotel, civic, and educational sectors.
Heather received her B.S. in Construction Engineering from the University of Akron. She is a LEED AP and serves as a community leader by dedicating her time and talent to support the National Association for Women in Construction (NAWIC), Women for Economic Leadership and Development (WELD) and the OSU CSM Industry Advisory Council.
Audra Smith currently works as a Project Executive for Pepper Construction and has extensive experience in construction management on large scale projects throughout central and northeastern Ohio. She has worked in the healthcare, higher education, mixed-use, and corporate office markets and has been involved in $578M worth of construction during her 15 year career. Her expertise is in client relationships and developing the appropriate processes, communication, and expectations to allow her project teams to be successful and meet the needs of the clients and owners. Prior to joining Pepper, she worked for Whiting-Turner Contracting and was responsible for all project interface, shutdown, and management of impactful construction to clients.
Audra earned a B.S. in Industrial Engineering from Pennsylvania State University and holds LEED AP BD+C credentials. She resides in Galena, Ohio with her sons Max and Vin. They share a passion for sports and her sons’ involvement in the Olentangy Local School District athletic programs afford her the opportunity to mentor, coach, and support their programs through fundraising and classroom academic activities.
Speaker: Dr. Patric Glynn, Stitch Fix; Data Scientist, Client Algorithms
Title: “Data Science: A Nontraditional Career Option that Combines Experimentation, Programming, Math, and Statistics“
Choosing whether to pursue a career in academia or industry is a big decision. For individuals who choose industry, there are many diverse job options, including research scientist, product engineer, and others. In recent years, an additional career choice has surfaced for those that possess skills in experimentation, programming, math, and statistics: Data Science.
In this talk, I will discuss how Data Science can be a viable, fulfilling career path for Biomedical Engineering students looking for a nontraditional industry role. Specifically, we will cover:
- What is Data Science?
- What do Data Scientists do?
- How do the skills and training in Biomedical Engineering apply to Data Science?
I will also cover how being successful at a high-growth silicon valley technology company requires a different project and experimentation approach when compared to traditional academic research.
Dr. Patric Glynn received his B.S. in Biomedical Engineering from Case Western Reserve University in 2009 and his Ph.D. in Biomedical Engineering from The Ohio State University in 2015. While working on his Ph.D., he received an American Heart Association Predoctoral Fellowship. Dr. Glynn worked as a Data Scientist at Nationwide Insurance before moving out to San Francisco, where he has spent the last 1.5 years as a Data Scientist at Stitch Fix. At Stitch Fix, he is a member of the Client Algorithms team, where he focuses on researching and implementing company strategies for algorithmic approaches to customer retention and reengagement.
Speaker: Dr. Matthew Becker, Associate Dean for Research; W. Gerald Austen Endowed Chair in Polymer Science and Polymer Engineering
Affiliation: University of Akron
Title: Building bone with polymers – How new materials and additive manufacturing are changing medicine
Recent synthetic advances have enabled the synthesis of polymers designed to elicit specific cellular functions and to direct cell-cell interactions. Motivated by traumatic injuries experienced by warfighters, we are developing novel materials and devices designed to repair segmental bone defect and achieve limb salvage. Biomimetic approaches based on polymers derivatized with adhesive receptor-binding peptides glycoproteins and tethered growth factors have been reported to enhance interactions at the biotic-synthetic interface. Further advances in both synthetic methodology and scaffold fabrication are needed to drive these efforts forward. This presentation will describe the use of several translationally relevant chemistries and functionalization strategies that are impacting the practice of medicine and how physicians are planning for future therapies that were not possible previously.
Matthew L. Becker is the W. Gerald Austen Endowed Chair of Polymer Science and Polymer Engineering and The Associate Dean for Research in the College of Polymer Science and Polymer Engineering at The University of Akron. His multidisciplinary research team is focused on developing bioactive polymers for regenerative medicine and addressing unmet medical needs at the interface of chemistry, material science and medicine. He is the founder of three start-up companies. He earned a PhD in organic chemistry at Washington University in St. Louis as an NIH Chemistry Biology Interface Training Fellow. In 2003, Dr Becker moved to the Polymers Division of the National Institute of Standards and Technology for a NRC Postdoctoral Fellowship. He joined the permanent staff as a project leader in 2005 before moving to The University of Akron in 2009. Professor Becker was awarded the ACS Publications Macromolecules-Biomacromolecules Young Investigator Award in 2015 and is a fellow of the ACS PMSE Division and the Royal Society of Chemistry.
Speaker: Dr. Yizhou Dong
Affiliation: The Ohio State University
Title: Development of nanomaterials for mRNA therapeutics and genome editing
Messenger RNA (mRNA) therapeutics have shown great promise for purpose of expressing functional proteins. However, the efficient and safe delivery of mRNA remains a key challenge for the clinical application of mRNA based therapeutics. Lipid and lipid-like nanoparticles possess the potential for mRNA delivery. Based on our previous experiences, we have designed and synthesized N1,N3,N5-tris(2-aminoethyl)benzene-1,3,5-tricarboxamide (TT). We applied an orthogonal experimental design to investigate the impacts of formulation components on delivery efficiency. TT3 LLNs, a lead material fully recovered the level of human factor IX (hFIX) to normal physiological values in FIX-knockout mice. In addition, we demonstrated that TT3 LLNs were capable of effectively delivering Cas9 mRNA and guide RNA to the mouse liver for genome editing.
Dr. Dong received his Ph.D. degree in pharmaceutical sciences from the University of North Carolina at Chapel Hill (UNC-CH) in 2009 under the supervision of Professor K.-H. Lee. He was a postdoctoral fellow from 2010-2014 in Children’s Hospital Boston at Harvard Medical School and also in the David H. Koch Institute for Integrative Cancer Research at MIT in the laboratory of Professors Robert Langer and Daniel Anderson. He also holds B.S. in pharmaceutical sciences from Peking University, Health Science Center (2002) and M.S. in organic chemistry from Shanghai Institute of Organic Chemistry (2005). He joined the Division of Pharmaceutics and Pharmaceutical Chemistry at The Ohio State University as Assistant Professor in 2014.